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Updated: Oct 1, 2026
Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
Published on: May 10, 2018
Rapid quantitative determination and assessment of insulin in oil formulation by micellar electrokinetic capillary
Bin Deng1, Zheng Liu, Guoan Luo
1Analytical Center, School of Life Science and Engineering, Tsinghua University, 100084, Beijing, People's Republic of China.
Abstract:
A rapid, simple and precise micellar electrokinetic capillary chromatrography (MEKC) with diode array detector had been developed to the determination of insulin in oil formulation. As the micelle-forming ampholytic surfactant, Labrasol was chosen for the separation and analysis of the hydrophobic insulin in oil formulation in this experiment. A buffer containing 10 mmol/ml Tris-HCl plus 10% acetonitrile (ACN) at pH 8.2 was used for running buffer. Samples and tested samples were successfully separated during 8 min with a good linear range(r>0.99) and a concentration detection limit of 0.01 mg/ml. Furthermore, quantitative determinations of insulin in oil formulation had been described and assessed after handled with different pre-treatment conditions to reveal the effect of pH, temperature and tryptic digestion.
