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Helicobacter typhlonius sp. nov., a Novel Murine Urease-Negative Helicobacter Species.

C L Franklin1, P L Gorelick, L K Riley

  • 1Research Animal Diagnostic and Investigative Laboratory, Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri 65211, USA.

Journal of Clinical Microbiology
|October 30, 2001
PubMed
Summary
This summary is machine-generated.

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A new urease-negative bacterium, Helicobacter typhlonius, was identified in laboratory mice. This novel Helicobacter species causes typhlocolitis in immunodeficient mice, adding to the list of mouse-colonizing pathogens.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Rodent Pathogens

Background:

  • Several Helicobacter species colonize rodents, with new species frequently identified using PCR.
  • Previously uncharacterized Helicobacter species have been isolated from rodent populations.

Purpose of the Study:

  • To describe a novel urease-negative Helicobacter species, Helicobacter typhlonius sp. nov., isolated from laboratory mice.
  • To characterize the pathogenicity and genetic relatedness of H. typhlonius.

Main Methods:

  • Isolation and characterization of a novel Helicobacter species from laboratory mice.
  • Infection studies in immunodeficient mice to assess pathogenicity.
  • Genetic analysis, including 16S rRNA gene sequencing.
  • Biochemical testing for urease, H(2)S, and indoxyl-acetate hydrolysis.

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Main Results:

  • Helicobacter typhlonius sp. nov. was isolated independently by two laboratories from mouse colonies.
  • Infection with H. typhlonius induced typhlocolitis in immunodeficient mice.
  • H. typhlonius is genetically related to H. hepaticus but distinct due to a large intervening sequence in its 16S rRNA gene.
  • Biochemically, H. typhlonius is urease-negative, H(2)S-negative, and indoxyl-acetate hydrolysis-negative.
  • H. typhlonius prevalence in rodents is lower than H. hepaticus and H. rodentium, but similar to H. bilis.

Conclusions:

  • Helicobacter typhlonius represents a newly identified urease-negative Helicobacter species colonizing laboratory mice.
  • H. typhlonius is capable of inducing typhlocolitis in immunodeficient mouse models.
  • This discovery expands the known diversity of Helicobacter species in mice and their potential to cause enteric disease.