Related Experiment Video
Updated: Oct 3, 2026
Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
Published on: March 10, 2020
Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in human stool
E Collins1, M Glennon, S Hanley
1The National Diagnostics Centre, BioResearch Ireland, National University of Ireland. Evelyn.Collins@nuigalway.ie
Abstract:
DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA probes specific for the Campylobacter genus, Campylobacter jejuni, and Campylobacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-positive specimens were PCR/DNA probe positive for C. jejuni. The assay is rapid and simple and can be applied to stool specimens for the detection of Campylobacter.
