Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A transcript encoding a nucleic acid-binding protein specifically expressed in maize seeds.

A Heyl1, J Muth, G Santandrea

  • 1MPI für Züchtungsforschung, Köln, Germany.

Molecular Genetics and Genomics : MGG
|October 31, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Causality and functional relevance of BRCA1 and BRCA2 pathogenic variants in non-high-grade serous ovarian carcinomas.

The Journal of pathology·2023
Same author

Case report of gastrointestinal localization of SARS-CoV-2 and open abdomen technique in an Italian emergency surgery department for gastrointestinal bleeding.

Annals of medicine and surgery (2012)·2021
Same author

Beam Spin Asymmetry in Semi-Inclusive Electroproduction of Hadron Pairs.

Physical review letters·2021
Same author

Beam-Target Helicity Asymmetry for γ[over →]n[over →]→π^{-}p in the N^{*} Resonance Region.

Physical review letters·2017
Same author

Effectiveness of Intraoperative Parathyroid Monitoring (ioPTH) in predicting a multiglandular or malignant parathyroid disease.

International journal of surgery (London, England)·2017
Same author

Single fraction volumetric modulated arc radiosurgery of brain metastases.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2015
Same journal

A CLN8 biallelic missense variant causes epilepsy with severe treatment-resistant psychosis.

Molecular genetics and genomics : MGG·2026
Same journal

Expression, prognostic value and function of LMBR1L in gastric cancer.

Molecular genetics and genomics : MGG·2026
Same journal

HIPK3 silencing promotes M2 macrophage polarization through NF-κB signaling pathway to enhance oral cancer progression.

Molecular genetics and genomics : MGG·2026
Same journal

Comparison of microbial profiles between hospital wastewater and river water.

Molecular genetics and genomics : MGG·2026
Same journal

Genetic landscape of the Brahmin population of Gujarat, India, using X-chromosome STR markers.

Molecular genetics and genomics : MGG·2026
Same journal

RUNX2 influences alternative splicing of genes associated with gastric cancer progression by regulating SF3B6 expression in HGC-27 cells.

Molecular genetics and genomics : MGG·2026
See all related articles

Researchers identified a novel maize protein, MEM1 (Maize Endosperm Motif binding protein), that binds nucleic acids and may play a role in seed development. This protein shows similarities to molecular chaperones.

Area of Science:

  • Plant molecular biology
  • Gene regulation
  • Protein biochemistry

Background:

  • Seed development involves complex gene expression regulation.
  • Molecular chaperones play critical roles in protein folding and cellular processes.
  • Novel proteins with potential regulatory functions are frequently discovered in plant endosperm.

Purpose of the Study:

  • To characterize a novel seed-specific protein, MEM1, from maize.
  • To investigate the DNA/RNA binding and dimerization properties of MEM1.
  • To explore the potential function of MEM1 in maize endosperm development.

Main Methods:

  • cDNA cloning and sequencing to identify the MEM1 gene.
  • Yeast one-hybrid system to assess transcriptional activation.

Related Experiment Videos

  • In vitro nucleic acid binding assays.
  • Recombinant protein expression and purification.
  • Native PAGE and co-immunoprecipitation for dimerization studies.
  • Subcellular and size fractionation.

    • Main Results:

    • A cDNA clone encoding MEM1, a novel protein family member related to DnaJ chaperones, was obtained.
    • MEM1 binds to the endosperm motif and activates transcription in yeast.
    • Recombinant MEM1 exhibits in vitro nucleic acid binding, with a preference for RNA.
    • MEM1 forms homodimers, dependent on a C-terminal domain.
    • MEM1 is expressed in mid- to late-term endosperm cells and localized to the cytosol.

    Conclusions:

    • MEM1 represents a novel class of plant proteins with potential roles in gene regulation.
    • Its nucleic acid binding and dimerization capabilities suggest involvement in RNA metabolism or protein complex formation.
    • MEM1 may contribute to endosperm and protein body development in maize.