Related Experiment Video
Updated: Aug 9, 2026
08:54
In Vivo Assessment of Alveolar Macrophage Efferocytosis Following Ozone Exposure
Published on: October 22, 2019
Effect of ozone on neutrophil function in vitro
M Margalit1, E Attias, D Attias
1Gaucher Clinic and the Department of Medicine and Surgery A, Shaare-Zedek Medical Center, Hadassah University Hospital (Mount Scopus), Jerusalem, Israel.
Clinical and Laboratory Haematology
|October 31, 2001
Summary
Ozone major autohemotherapy, a complementary medicine, showed no adverse effects on neutrophil function in vitro. This suggests the procedure is safe for blood cells, supporting further clinical trials for efficacy.
Area of Science:
- Alternative Medicine
- Immunology
- Hematology
Background:
- Ozone therapy, particularly major autohemotherapy, is used in alternative medicine for various conditions.
- Ozone's effects on cytokines and inflammatory responses are known, necessitating safety evaluation.
- Neutrophil function is critical in immune response and can be affected by external agents.
Purpose of the Study:
- To investigate the in vitro effects of ozone administration on peripheral blood neutrophil function.
- To assess safety of ozone major autohemotherapy concerning neutrophil function at commonly used concentrations.
Main Methods:
- Blood from healthy volunteers was exposed ex vivo to ozone (30 microg/ml).
- Neutrophil adhesion, chemotaxis, and oxygen radical (O-2) production were measured before and after ozone exposure.
- Comparison of functional parameters between ozone-exposed and unexposed neutrophils.
Main Results:
- No significant differences were observed in neutrophil adhesion and chemotaxis.
- A minimal, clinically insignificant decrease in O-2 production was noted post-ozone exposure (P=0.01).
- Neutrophil function remained largely unaffected by ozone treatment in vitro.
Conclusions:
- Ozone major autohemotherapy is confirmed to be safe regarding peripheral blood neutrophil function.
- The findings support the safety of this procedure for blood cell populations.
- Encourages well-designed clinical trials to evaluate the therapeutic efficacy of ozone major autohemotherapy.
