Related Experiment Video
Updated: Jul 12, 2026

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
Published on: June 25, 2015
Nucleotide requirements for CDX2 binding to the cis promoter element mediating intestine-specific expression of
M D Di Guglielmo1, J Park, S Schulz
1Division of Clinical Pharmacology, Departments of Medicine, Biochemistry and Molecular Pharmacology, Thomas Jefferson University, 812 Medical Office Building, 1100 Walnut Street, Philadelphia, PA 19107, USA. mdd002@jefferson.edu
Abstract:
Guanylyl cyclase C (GC-C), specifically expressed by intestinal epithelial cells, is the receptor for the Escherichia coli heat-stable enterotoxin that causes diarrhea. Tissue-specific expression of GC-C is mediated by the intestinal transcriptional regulator CDX2. This trans-activating protein regulates intestine-specific expression by binding to a critical sequence in the proximal promoter of GC-C. The precise nucleotide elements mediating CDX2 binding to promoter elements remain undefined. Several nuclear proteins form complexes with a DNA probe containing the promoter element of GC-C mediating CDX2 binding. The present study examined the nucleotide requirements in the consensus binding site and flanking regions in the cis element that mediates specific CDX2 binding to the promoter of GC-C. These studies identified seven core base pairs in the critical promoter element mediating tissue-specific expression of GC-C that are required for CDX2 binding. In addition, base pairs flanking this core sequence contribute to and are required for CDX2 recognition. These studies describe the precise nucleotide sequence within the GC-C promoter that comprises the CDX2 binding site required for intestine-specific expression.
More Related Videos
09:58An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
Published on: November 29, 2016
06:21An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
Published on: May 7, 2018
Related Concept Videos
Cell Specific Gene Expression
Cis-regulatory Sequences
Cooperative Binding of Transcription Regulators
The Eukaryotic Promoter Region
Cis-regulatory Sequences
GPCRs Regulate Adenylyl Cylase Activity
Two...