Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genomic context modulates insulator activity through promoter competition.

H N Cai1, Z Zhang, J R Adams

  • 1Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA. hcai@arches.uga.edu

Development (Cambridge, England)
|October 31, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Analysis of pathogenic characteristics in cases of hepangina in sentinel hospitals in Yancheng City from 2023 to 2024].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2026
Same author

[Pediatric Ewing sarcoma in the rare sites: a clinicopathological analysis of eight cases].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2026
Same author

[Advances in biomarkers for delayed encephalopathy after acute carbon monoxide poisoning].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2025
Same author

[Association of triglyceride-glucose index and cardiovascular disease in a community-based Chinese cohort].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2025
Same author

[Distribution of respiratory pathogens in patients with pneumonia in Yinzhou, Ningbo, 2015-2024].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2025
Same author

[Analysis on current status and outcomes of comprehensive control of cardiovascular disease risk factors based on community population cohort].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2025
Same journal

Retinoic acid and FGF signaling interact to control elongation and lineage specification in a mouse gastruloid model.

Development (Cambridge, England)·2026
Same journal

Expanding the C. elegans toolkit with gonad explants.

Development (Cambridge, England)·2026
Same journal

Nuclear Factor Y controls nutrient-adaptive epithelial growth by regulating mTOR in the Drosophila midgut.

Development (Cambridge, England)·2026
Same journal

Primordial germ cells differentially contribute to the germline in zebrafish.

Development (Cambridge, England)·2026
Same journal

Dissecting planar and vertical organiser signals in early chick neural development.

Development (Cambridge, England)·2026
Same journal

Real-time transcriptomic profiling of hPSC-derived cartilage during development identifies a key role for the extracellular matrix in homeostasis and protection.

Development (Cambridge, England)·2026
See all related articles

Chromatin insulators like suHw exhibit selective gene regulation. Their effectiveness depends on promoter competition for enhancers, revealing context-dependent mechanisms in gene organization.

Area of Science:

  • * Molecular Biology
  • * Genetics
  • * Developmental Biology

Background:

  • * Chromatin insulators are crucial for regulating gene expression by preventing aberrant enhancer-promoter interactions.
  • * Previous research indicated that insulator activity is not absolute but rather quantitative and selective.
  • * The study focuses on the suHw insulator in transgenic Drosophila to understand its regulatory mechanisms.

Purpose of the Study:

  • * To investigate the factors and mechanisms governing the effectiveness of the suHw insulator.
  • * To determine how promoter competition influences insulator activity.
  • * To explore the role of cis-context in modulating insulator function.

Main Methods:

  • * Investigated the suHw insulator's function in transgenic Drosophila.

Related Experiment Videos

  • * Analyzed the blockage of the AE1 enhancer from a downstream promoter.
  • * Assessed the impact of competing promoters on insulator effectiveness.

    • Main Results:

    • * suHw-mediated blockage of the AE1 enhancer is inversely related to the promoter's ability to compete for the enhancer.
    • * Insulator effectiveness varies based on the properties of neighboring promoters, with highly competitive neighbors enhancing blockage.
    • * Promoter competition for enhancers, influenced by core promoter sequences, dictates insulator effectiveness.

    Conclusions:

    • * Insulator effectiveness is modulated by promoter competition, suggesting a gene organization mechanism.
    • * The context-dependent nature of insulator function provides regulatory flexibility.
    • * Insulators impose organizational constraints on eukaryotic gene complexes.