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Updated: Aug 12, 2026
Ex Vivo Corneal Organ Culture Model for Wound Healing Studies
Published on: February 15, 2019
Epithelial plugs in radial keratotomy: the origin of incisional keratitis?
J Bergmanson1, E Farmer, J Goosey
1Texas Eye Research and Technology Center, University of Houston, Houston, Texas, USA. jbergmanson@UH.edu
Purpose:
Incisions in radial keratotomy (RK) patients were examined histopathologically to explore the cause of delayed infections occurring following this procedure.
Methods:
Four corneas from two successful RK patients were obtained postmortem and 1 cornea from an unsuccessful RK was collected following lamellar keratoplasty. The tissue was prepared for light and transmission electron microscopy.
Results:
All incisions examined contained an epithelial plug. The average depth of the plugs for left and right corneas in the 2 successful cases were 142.58 microm (range: 36.46-183.04 microm) and 58.44 microm (range: 29.27-115.66 microm), whereas the unsuccessful case these measurements were 121.06 microm (range: 70.03-172.01 microm). In the successful cases, the plugs were on average 4.2 cells deep (range: 2-5) in one case and for the other the plugs were 8.78 cells deep (range: 1.5-11.5) and 2.72 cells wide (range: 2-4). In the unsuccessful case the plugs were on average 6.89 cells deep (range: 5-11) and 8.56 cells wide (range: 4-13). The average epithelial thickness, measured 200 microm on either side away from the plug, was 47.11 microm and 55.09 microm for the successful cases, and 27.52 microm for the unsuccessful case. Degenerate cells were often observed within the plug and along the overlying surface. Lamellae cut during surgery remained severed in all corneas observed. The stroma adjacent to the incision contained an elevated number of stromal cells (8%-78%).
Conclusions:
Healing did not include repair of anterior limiting lamina or stromal lamellae, which all remained severed in all incisions observed. Epithelial plugs were invariably present in all incisions regardless of clinical outcome, thus, increasing the distance a basal cell travels to reach the epithelial surface by 2 to 4 times. Since the epithelial cell has a limited life, it is hypothesized that cells originating in the plug may die before reaching the surface, thus, leading to intermittent loss of surface integrity. The loss of the epithelial barrier allows invasion of microorganisms and the delayed onset of keratitis. The larger the plug the greater the possibility of surface defects and resultant infection.
