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Related Experiment Videos

Serum testosterone determination by mass fragmentography.

I Björkhem, O Lantto, L Svensson

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |April 2, 1975
    PubMed
    Summary
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    A new mass fragmentographic method accurately measures testosterone in plasma and serum. This technique confirms diurnal testosterone secretion rhythms, offering a reliable alternative to radioimmunoassay.

    Area of Science:

    • Endocrinology
    • Analytical Chemistry
    • Mass Spectrometry

    Background:

    • Accurate testosterone measurement is crucial for diagnosing endocrine disorders.
    • Existing methods like radioimmunoassay may have limitations in specificity or require extensive validation.

    Purpose of the Study:

    • To develop and validate a mass fragmentographic reference method for quantifying testosterone in biological samples.
    • To compare the performance of this new method against established radioimmunoassay techniques.

    Main Methods:

    • Addition of a known amount of [4--14C]testosterone to serum samples.
    • Ether extraction and thin-layer chromatography for purification.
    • Derivatization to di-trimethylsilyl esters.
    • Analysis using gas chromatography-mass spectrometry with multiple ion detection.

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    Main Results:

    • The mass fragmentographic method demonstrated a relative standard deviation of approximately 2.7%.
    • A strong correlation (regression coefficient ~0.83) was observed when compared to radioimmunoassay.
    • The method successfully confirmed the diurnal rhythm of testosterone secretion.

    Conclusions:

    • Mass fragmentography provides a precise and reliable reference method for serum testosterone determination.
    • This technique offers a valuable alternative or complementary tool to radioimmunoassay for clinical and research applications.
    • The study reaffirms the presence of diurnal variations in testosterone secretion using a novel analytical approach.