Culture of Zollinger-Ellison tumor cells

We have successfully grown six Zollinger-Ellison tumors in vitro with use of a monlayer tissue culture technique. The initial gastrin concentration in the medium varied between 0 and 100 ng per ml. Many cytoplasmic secretory granules were seen in the cells of one culture population. Gastrin secretion was stimulated by the addition of fresh medium to the culture flasks. Both the culture cells and the medium were found to contain primarily big gastrin (G-34) but smaller amounts of little gastrin (G-17) were also present. Gastrin concentration in the medium decreased with time in culture until no hormone was detected between 2 and 6 weeks, possibly because of endocrine cell dedifferentiation and an increased proportion of fibroblasts in the population.