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TAF(II)250: a transcription toolbox.

D A Wassarman¹, F Sauer

¹National Institutes of Health, National Institute of Child Health and Human Development, Cell Biology and Metabolism Branch, Bethesda, MD 20892, USA.

Journal of Cell Science

|November 1, 2001

Summary

TAF(II)250 is a key coactivator that helps gene activators recruit transcription machinery to DNA. It stabilizes transcription factor TFIID binding and modifies chromatin, enabling RNA polymerase II transcription initiation.

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Area of Science:

Molecular Biology
Gene Regulation
Biochemistry

Background:

RNA polymerase II transcription initiates gene expression.
Transcriptional activation involves dynamic chromatin changes and general transcription factor (GTF) assembly.
TFIID, a GTF, interacts with DNA-bound activators via TATA-box binding protein (TBP)-associated factors (TAFs).

Purpose of the Study:

To elucidate the multifaceted roles of TAF(II)250 in transcriptional activation.
To propose a model for how TAF(II)250 converts activation signals into transcription.

Main Methods:

The study focuses on the biochemical activities and functions of TAF(II)250.
Analysis of TAF(II)250's interactions with activators, TBP, core promoter elements, and histones.
Investigation of TAF(II)250's enzymatic activities (kinase, acetylase, ubiquitin-activating/conjugating).

Main Results:

TAF(II)250 acts as a scaffold for TFIID assembly.
It binds gene activators, recruiting TFIID to promoters.
TAF(II)250 regulates TBP binding, interacts with promoter elements, and binds acetylated histones.
It possesses enzymatic activities modifying histones and GTFs.

Conclusions:

TAF(II)250 stabilizes TFIID at promoters and modifies chromatin structure.
These actions facilitate GTF assembly and enable transcription initiation.
A model is proposed for TAF(II)250's role in signal transduction for RNA polymerase II transcription.

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