Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Shiga-toxin-converting bacteriophages.

H Schmidt1

  • 1Institut für Hygiene und Mikrobiologie der Universität Würzburg, Germany. hschmidt@hygiene.uni-wuerzburg.de

Research in Microbiology
|November 1, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The protein sequence and some intron positions are conserved between the switching gene swi10 of Schizosaccharomyces pombe and the human excision repair gene ERCC1.

Nucleic acids research·1992
Same author

Characterization of a novel enzyme, N6-acetyl-L-lysine: 2-oxoglutarate aminotransferase, which catalyses the second step of lysine catabolism in Candida maltosa.

Antonie van Leeuwenhoek·1992
Same author

Modulation of the glucagon-dependent activation of the phosphoenolpyruvate carboxykinase gene by oxygen in rat hepatocyte cultures. Evidence for a heme protein as oxygen sensor.

FEBS letters·1992
Same author

Molecular analysis of nosocomial infection by oxacillin-resistant Staphylococcus aureus lacking protein A and clumping factor.

Lancet (London, England)·1992
Same author

Mdr1/P-glycoprotein, topoisomerase, and glutathione-S-transferase pi gene expression in primary and relapsed state adult and childhood leukaemias.

British journal of cancer·1992
Same author

[Surfactant administration in acute respiratory failure].

Der Anaesthesist·1992
Same journal

Deletion of major shell proteins of ethanolamine utilization microcompartment reduces intrinsic antibiotic resistance, biofilm, and intracellular survival of Salmonella Typhimurium.

Research in microbiology·2025
Same journal

Dynamic exometabolomics reveals metabolic adaptations of Staphylococcus epidermidis to pH-mimicking skin and bloodstream.

Research in microbiology·2025
Same journal

Co-expression network insights into Leptospira interrogans pathogenesis.

Research in microbiology·2025
Same journal

Differential DNA methylation associated with virulence attenuation in Edwardsiella piscicida.

Research in microbiology·2025
Same journal

Klebsiella pneumoniae-induced pneumonia: Pathogenesis, immune interactions, and antimicrobial resistance in a global context.

Research in microbiology·2025
Same journal

Investigating inhibitory effect of sterol targeting compounds against B. anthracis: Membrane microdomain a probable target.

Research in microbiology·2025
See all related articles

Shiga toxins (Stx), potent cytotoxins causing severe human disease, are primarily produced by Escherichia coli and Shigella dysenteriae. Stx-converting bacteriophages (Stx phages) play a crucial role in Stx expression and gene transfer, contributing to new STEC variants.

Area of Science:

  • Microbiology
  • Bacteriology
  • Genetics

Background:

  • Shiga toxins (Stx) are potent cytotoxins responsible for severe human diseases.
  • Stx are primarily produced by Escherichia coli and Shigella dysenteriae type 1.
  • The genes encoding Stx are typically found within the genomes of lambdoid prophages, known as Stx-converting bacteriophages (Stx phages).

Purpose of the Study:

  • To investigate the role of Stx-converting bacteriophages in the expression and dissemination of Shiga toxins.
  • To understand the genetic location and regulatory mechanisms controlling Stx production.
  • To elucidate the contribution of Stx phages to the emergence of new Shiga toxin-producing E. coli (STEC) variants.

Main Methods:

  • Bioinformatic analysis of Stx phage genomes.

Related Experiment Videos

  • Gene expression studies focusing on the late region of the phage genome.
  • Investigation of phage induction and transduction processes.

    • Main Results:

    • Stx genes are located in a conserved position within the late region of Stx phage genomes, downstream of the PR' promoter.
    • The location suggests regulation by a Q-like antiterminator, implying phage induction triggers Stx production.
    • Stx phages are capable of in vivo and in vitro transduction, facilitating lateral gene transfer.

    Conclusions:

    • Stx phages are critical for Shiga toxin expression and dissemination.
    • Phage induction is a key event in activating Stx production.
    • Stx phages significantly contribute to the emergence and evolution of STEC through lateral gene transfer.