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Updated: Oct 3, 2026

Imaging Subcellular Structures in the Living Zebrafish Embryo
Published on: April 2, 2016
Antibody fusions with fluorescent proteins: a versatile reagent for profiling protein expression
K Morino1, H Katsumi, Y Akahori
1Institute for Antibody, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
Abstract:
We developed a system by which antibodies, fused to fluorescent proteins with different wavelengths, can be prepared within a month against various antigens. An antibody library composed of a large number of single-chain Fv-CL fragment was constructed by means of a phage-display system. The constructs were designed to facilitate changing of the protein forms by simple enzyme manipulation. In the present study, we adopted a molecular form of antibody in which a single-chain Fv-CL fragment is fused with a green fluorescent protein (GFP) or red fluorescent protein (RFP). In addition, a His-tag was inserted between CL and GFP (or RFP). We describe the utility of this system using Caenorhabditis elegans embryo as a model.
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