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Published on: January 17, 2016
Cloning and characterization of a chicken platelet-derived growth factor B-chain cDNA
H Horiuchi1, T Inoue, S Furusawa
1Department of Immunobiology, Faculty of Applied Biological Science, Hiroshima University, Kagamiyama, 739-8528, Higashi-Hiroshima, Japan. hhori10@hiroshima-u.ac.jp
Abstract:
Avian thrombocytes are nucleated blood cells homologous in function to mammalian platelets. In the present study, we obtained a cDNA from chicken thrombocyte polyadenylated RNA [Poly(A)+RNA], which coded for the chicken PDGF-B chain. The sequence was 1083-bp long and had an open reading frame (ORF) of 753-bp. At the amino acid level, the predicted mature protein showed 69% homology with the processed coding region of human PDGF-B. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showed that PDGF-B mRNA was expressed at high levels in thrombocytes and in the lung. The expression of PDGF-B chain mRNA in thrombocytes reached its maximum level 12h following type 1 collagen treatment. These results suggest that chicken PDGF-B chain may play an important role in the vascular system and in healing wounded tissue.
