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Published on: January 22, 2018
A novel Rtg2p activity regulates nitrogen catabolism in yeast
M M Pierce1, M L Maddelein, B T Roberts
1Laboratory of Biochemistry and Genetics, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Building 8, Room 225, 8 Center Drive MSC0830, Bethesda, MD 20892-0830, USA.
The Rtg2 protein in yeast regulates nitrogen metabolism, enabling ureidosuccinate uptake independently of the [URE3] prion. This reveals a novel function for Rtg2p in the nitrogen catabolism pathway.
Area of Science:
- * Molecular and Cellular Biology
- * Yeast Genetics
- * Nitrogen Metabolism Regulation
Background:
- * The Ure2p protein and the [URE3] prion influence DAL5 transcription and ureidosuccinate (USA+) uptake in Saccharomyces cerevisiae.
- * Rtg2p facilitates the nuclear entry of Rtg1 and Rtg3 proteins, regulating glutamate-repressible genes.
Purpose of the Study:
- * To investigate the role of Rtg2p in the USA+ phenotype, particularly its relationship with the [URE3] prion.
- * To elucidate the novel function of Rtg2p in nitrogen catabolism regulation.
Main Methods:
- * Analysis of USA+ phenotype in various yeast mutant strains (rtg2 Delta, rtg1 Delta, rtg3 Delta, mks1 Delta).
- * Assessment of DAL5 gene expression and GLN3 dependence.
- * Investigation of the effect of glutamate and Ure2p overexpression.
Main Results:
- * rtg2 Delta yeast strains exhibit the USA+ phenotype even without the [URE3] prion, indicating a regulatory effect independent of prion formation.
- * The USA+ phenotype in rtg2 Delta strains is dependent on GLN3 and causes DAL5 overexpression.
- * The phenotype is blocked by mks1 Delta mutations but not by Ure2p overexpression.
Conclusions:
- * Rtg2p plays a novel role in regulating ureidosuccinate uptake, separate from its known function in nuclear transport.
- * Rtg2p appears to act upstream in the nitrogen catabolism regulatory pathway, influencing DAL5 transcription via GLN3.
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