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Published on: October 6, 2018
Echinococcus multilocularis in European wolves (Canis lupus)
K Martínek1, L Kolárová, E Hapl
1Department of Biology, University of West Bohemia, Pilsen, Czech Republic. kmartin@kbi.zcu.cz
Parasitology Research
|November 2, 2001
Summary
Echinococcus multilocularis tapeworm DNA was detected in Slovakian wolves using nested PCR. This finding expands the known range of this parasite
Area of Science:
- Veterinary Parasitology
- Wildlife Disease Ecology
- Molecular Diagnostics
Background:
- Echinococcus multilocularis is a tapeworm with significant public health implications.
- Understanding its host range and distribution is crucial for effective control strategies.
- Protected carnivore populations may act as reservoirs for Echinococcus multilocularis.
Purpose of the Study:
- To investigate the presence of Echinococcus multilocularis in protected carnivore species in Slovakia.
- To determine the prevalence and distribution of Echinococcus multilocularis DNA in faecal samples.
- To identify potential definitive hosts for Echinococcus multilocularis in the study area.
Main Methods:
- Nested PCR assay was employed for sensitive DNA detection.
- Faecal samples were collected from wolves, bears, and lynx in two Slovakian mountain ranges.
- DNA extraction and amplification targeted specific sequences of Echinococcus multilocularis.
Main Results:
- Echinococcus multilocularis DNA was detected in 3 out of 31 wolf faecal samples examined.
- Positive samples originated from both the Muránska Planina and Bukovské Vrchy mountains.
- No Echinococcus multilocularis DNA was found in faecal samples from bears or lynx.
Conclusions:
- Wolves in Slovakia are confirmed definitive hosts for Echinococcus multilocularis.
- The study extends the known geographical range and host spectrum of Echinococcus multilocularis in Europe.
- Continued monitoring of wildlife populations is recommended for parasite surveillance.
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