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CD147 is a signaling receptor for cyclophilin B
V Yurchenko1, M O'Connor, W W Dai
1The Picower Institute for Medical Research, Manhasset, New York 11030, USA.
Biochemical and Biophysical Research Communications
|November 2, 2001
Summary
CD147 acts as a cell-surface receptor for both cyclophilin A (CyPA) and cyclophilin B (CyPB). This finding reveals CD147
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Cyclophilins A and B (CyPA and CyPB) are secreted proteins involved in inflammatory responses.
- CD147 was previously identified as a cell-surface receptor for CyPA, mediating ERK activation and chemotaxis.
- The role of CD147 as a receptor for CyPB was not previously established.
Purpose of the Study:
- To investigate whether CD147 also functions as a receptor for CyPB.
- To elucidate the functional consequences of CyPB binding to CD147.
Main Methods:
- Utilized CD147-transfected Chinese hamster ovary (CHO) cells to assess CyPB-induced calcium (Ca2+) flux and chemotaxis.
- Employed antibodies against CD147 to block CyPB-mediated chemotaxis in primary human neutrophils.
Main Results:
- CD147-transfected CHO cells exhibited Ca2+ flux and chemotaxis in response to CyPB.
- Control CHO cells did not show these responses, confirming CD147 specificity.
- Antibodies targeting CD147 significantly inhibited the chemotactic response of human neutrophils to CyPB.
Conclusions:
- CD147 serves as a functional cell-surface receptor for extracellular cyclophilin B (CyPB).
- These findings extend the known function of CD147 to include binding and signaling for both CyPA and CyPB.
- CD147 is a key receptor mediating the biological effects of extracellular cyclophilins.
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