Transfected cell lines as tools for high throughput screening: a call for standards

Area of Science:

• Biomolecular Screening
• Assay Development
• Cell Line Engineering

Background:

• High-throughput screening (HTS) faces global challenges in target identification and library size.
• Stably transfected cell lines are increasingly vital tools in HTS strategies.
• These cell lines offer powerful assay design capabilities but introduce complex variables.

Purpose of the Study:

• To address the critical issue of using stably transfected cell lines in HTS.
• To advocate for the development of guidelines for cell line nomenclature and use.
• To enhance the reliability and reproducibility of HTS assays.

Main Methods:

• Perspective-based discussion of current HTS practices.
• Analysis of the role and challenges of transfected cell lines in screening.
• Literature review of existing standards and best practices.

Main Results:

• Stably transfected cell lines present both advantages and complexities in HTS.
• Lack of standardized definitions and usage hinders optimal application.
• The need for clear guidelines is evident for advancing HTS.

Conclusions:

• Development of nomenclature and usage guidelines for transfected cell lines is essential.
• Standardization will improve the robustness and interpretability of HTS data.
• Implementing these guidelines will benefit the broader HTS community.