Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bisphenol a binds to the low-affinity estrogen binding site.

W Washington1, L Hubert, D Jones

  • 1Department of Chemistry and The Health Research Center-Southern University, Southern University and A&M College, Baton Rouge, Louisiana 70813, USA.

In Vitro & Molecular Toxicology
|November 2, 2001
PubMed
Summary

Environmental estrogens like bisphenol A (BPA) may impact reproductive health. BPA preferentially binds to the Type II estrogen binding site (EBS), suggesting a new pathway for endocrine disruption.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combination of lovastatin, enalapril, and colchicine does not prevent restenosis after percutaneous transluminal coronary angioplasty.

The American journal of cardiology·1995
Same author

Caenorhabditis elegans UBC-1, a ubiquitin-conjugating enzyme homologous to yeast RAD6/UBC2, contains a novel carboxy-terminal extension that is conserved in nematodes.

DNA and cell biology·1995
Same author

Phase II trial of isotretinoin and recombinant interferon alfa-2a in metastatic colorectal carcinoma.

American journal of clinical oncology·1995
Same author

Characterization of a 17-kilodalton antigen of Bartonella henselae reactive with sera from patients with cat scratch disease.

Journal of clinical microbiology·1995
Same author

A portable regulatory element directs specific expression of the Caenorhabditis elegans ubiquitin gene ubq-2 in the somatic gonad.

Developmental biology·1995
Same author

Characterization of a novel 350-kilodalton nuclear phosphoprotein that is specifically involved in mitotic-phase progression.

Molecular and cellular biology·1995

Area of Science:

  • Endocrinology
  • Environmental Health
  • Toxicology

Background:

  • Environmental estrogens are linked to reproductive issues, including decreased male fertility and increased female breast cancer.
  • These effects are often attributed to interactions with estrogen receptors alpha and beta.
  • The role of the low-affinity Type II estrogen binding site (EBS) in mediating environmental estrogen effects is less understood.

Purpose of the Study:

  • To investigate if the Type II estrogen binding site (EBS) mediates the effects of environmental estrogens, specifically bisphenol A (BPA).
  • To analyze the binding affinity of BPA to the Type II EBS and compare it with estradiol and other environmental estrogens.

Main Methods:

  • Utilized a competition assay to analyze the binding of BPA to an enriched fraction of rat uterine Type II EBS.

Related Experiment Videos

  • Compared the binding affinity of BPA to Type II EBS with its affinity for estrogen receptor alpha.
  • Assessed the specificity of BPA binding to Type II EBS using endosulfan as a control environmental estrogen.

    • Main Results:

    • Bisphenol A (BPA) demonstrated the ability to compete with estradiol for binding to the Type II estrogen binding site (EBS).
    • At concentrations relevant to uterine proliferation, BPA inhibited estradiol binding to Type II EBS by over 50%.
    • BPA exhibited a preferential binding to the Type II EBS over estrogen receptor alpha, with an affinity only 8-10 times lower than diethylstilbestrol.

    Conclusions:

    • The Type II estrogen binding site (EBS) is a potential mediator for environmental estrogens like bisphenol A (BPA).
    • BPA's preferential binding to Type II EBS suggests a specific mechanism for its endocrine-disrupting activity.
    • These findings highlight the importance of considering Type II EBS in understanding the reproductive health impacts of environmental estrogens.