Related Experiment Videos
Vaccination with attenuated simian immunodeficiency virus by DNA inoculation
1Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia. skent@unimelb.edu.au
Journal of Virology
|November 2, 2001
Abstract:
Delivering attenuated lentivirus vaccines as proviral DNA would be simple and inexpensive. Inoculation of macaques with wild-type simian immunodeficiency virus strain mac239 (SIV(mac239)) DNA or SIV(mac239) DNA containing a single deletion in the 3' nef-long terminal repeat overlap region (nef/LTR) led to sustained SIV infections and AIDS. Injection of SIV(mac239) DNA containing identical deletions in both the 5' LTR and 3' nef/LTR resulted in attenuated SIV infections and substantial protection against subsequent mucosal SIV(mac251) challenge.