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Related Experiment Videos

Altered TRPC7 gene expression in bipolar-I disorder.

I S Yoon1, P P Li, K P Siu

  • 1Section of Biochemical Psychiatry, Centre for Addiction and Mental Health-Clarke Site, Toronto, Ontario, Canada.

Biological Psychiatry
|November 3, 2001
PubMed
Summary

Reduced TRPC7 gene expression is linked to calcium (Ca2+) imbalance in some bipolar disorder patients. This finding may indicate a specific trait in bipolar disorder pathophysiology.

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Area of Science:

  • Neuroscience
  • Genetics
  • Biochemistry

Background:

  • Altered calcium (Ca2+) homeostasis is implicated in bipolar disorder (BD).
  • Storage-operated calcium entry (SOCE) plays a role in cellular Ca2+ regulation.
  • TRPC7 and SERCA2s are proteins involved in SOCE.

Purpose of the Study:

  • To investigate TRPC7 and SERCA2s expression in B lymphoblast cell lines (BLCLs) from BD patients.
  • To determine the relationship between TRPC7/SERCA2s expression and intracellular Ca2+ levels in BD.

Main Methods:

  • Comparative reverse transcriptase-polymerase chain reaction to quantify mRNA levels.
  • Ratiometric spectrophotometry with Fura-2 to measure basal intracellular Ca2+ concentration ([Ca2+]B).
  • Analysis of BLCLs from BD-I, BD-II, major depressive disorder patients, and healthy subjects.

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Main Results:

  • TRPC7 mRNA levels were significantly lower in BD-I patients with high [Ca2+]B compared to other groups.
  • A significant inverse correlation was observed between TRPC7 mRNA levels and [Ca2+]B in the overall BD-I group.
  • No significant differences were noted for SERCA2s expression (data not shown).

Conclusions:

  • Reduced TRPC7 gene expression may be a characteristic feature in a subset of BD-I patients.
  • This reduction could contribute to disturbances in calcium homeostasis observed in bipolar disorder.
  • TRPC7 warrants further investigation as a potential biomarker for BD pathophysiology.