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Neurocalcin-actin interaction.

D Mornet1, A Bonet-Kerrache

  • 1Muscles et Pathologies, INSERM U128, IFR 24, Institut Bouisson-Bertrand, 778, Rue de la Croix Verte, 34196 Cedex 5, Montpellier, France.

Biochimica Et Biophysica Acta
|November 3, 2001
PubMed
Summary
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Myristoylated neurocalcin, a neuronal calcium sensor, binds directly to actin in a calcium-dependent way. This interaction may influence the rod outer segment-guanylate cyclase 1-neurocalcin signaling pathway.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Neuroscience

Background:

  • Neurocalcin is an N-myristoylated calcium-binding protein.
  • It belongs to a novel family of neuronal calcium sensors.

Purpose of the Study:

  • To investigate the interaction between myristoylated neurocalcin and actin.
  • To determine if this interaction is calcium-dependent.
  • To explore the potential functional implications of this interaction.

Main Methods:

  • Cosedimentation assays
  • Co-immunoprecipitation
  • EDC cross-linking using IAEDANS-labeled actin and neurocalcin-specific antibodies.

Main Results:

  • Myristoylated neurocalcin directly interacts with actin.

Related Experiment Videos

  • This interaction is dependent on the presence of calcium.
  • A novel 64 kDa complex of actin and neurocalcin was identified.

    • Conclusions:

    • Neurocalcin interacts with actin in a calcium-dependent manner.
    • This interaction may modulate the activity of the rod outer segment-guanylate cyclase 1-neurocalcin complex.