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Published on: February 21, 2015
Human APC2 localization and allelic imbalance.
C R Jarrett1, J Blancato, T Cao
1The Lombardi Cancer Research Center, Georgetown University School of Medicine, Washington, DC 20007, USA.
Cancer Research
|November 3, 2001
Summary
The adenomatous polyposis coli (APC) gene APC2 is located on chromosome 19p13.3 and shows allelic imbalance in ovarian cancers, suggesting it may be a tumor suppressor gene.
Area of Science:
- Genetics
- Molecular Biology
- Oncology
Background:
- A second adenomatous polyposis coli (APC)-like gene, APC2/APCL, has been identified and mapped to chromosome 19.
- The APC2 gene is located in a chromosomal region frequently lost in various cancers, notably ovarian cancer.
Purpose of the Study:
- To fine-map the APC2 gene to a specific chromosomal region.
- To investigate the potential role of APC2 as a tumor suppressor gene in ovarian cancer.
- To determine the subcellular localization and interactions of APC2 in ovarian cancer cells.
Main Methods:
- Fine mapping of APC2 using genetic markers.
- Interphase fluorescence in situ hybridization (FISH) to assess allelic imbalance in ovarian cancers.
- Overexpression of APC2 in SKOV3 ovarian cancer cells and immunofluorescence microscopy to determine localization.
- Treatment with cytochalasin D to assess APC2 association with actin filaments.
Main Results:
- APC2 was fine-mapped to chromosome 19p13.3, encompassing markers D19S883 and WI-19632.
- Nineteen out of twenty ovarian cancers screened showed APC2 allelic imbalance.
- Exogenously expressed APC2 localized to the Golgi apparatus, actin structures, and microtubules in SKOV3 cells.
- Endogenous APC2 colocalized with the Golgi apparatus and actin filaments, remaining associated even after actin disruption.
Conclusions:
- APC2 is a potential tumor suppressor gene in ovarian cancer due to its location and allelic imbalance.
- APC2 is involved in actin-associated cellular events, potentially influencing cell motility and adhesion.
- APC2 may function independently or with APC to regulate beta-catenin signaling.
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