Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Temperature-modulated array high-performance liquid chromatography.

A Premstaller1, W Xiao, H Oberacher

  • 1Stanford Genome Technology Center, Palo Alto, CA 94304, USA.

Genome Research
|November 3, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

COVID-19 and lockdown: effects on coercion and length of stay for patients with personality disorders.

Journal of psychiatric research·2025
Same author

Effect of coercive measures on mental health status in adult psychiatric populations: a nationwide trial emulation.

Epidemiology and psychiatric sciences·2024
Same author

Author Correction: A fast radio burst source at a complex magnetized site in a barred galaxy.

Nature·2022
Same author

Publisher Correction: A repeating fast radio burst associated with a persistent radio source.

Nature·2022
Same author

A fast radio burst source at a complex magnetized site in a barred galaxy.

Nature·2022
Same author

BITES: balanced individual treatment effect for survival data.

Bioinformatics (Oxford, England)·2022
Same journal

Complete sequencing of medaka genomes reveals the architecture of centromeric satellites, giant mobile elements, and sex chromosomes.

Genome research·2026
Same journal

Convergence and conflict among telomere specialized transposons across 60 million years of Drosophilid evolution.

Genome research·2026
Same journal

A unified analysis of cell type- and trajectory-associated pathways in single-cell data using Phoenix.

Genome research·2026
Same journal

Resf1 is required for proper placental development and configuration of trophoblast cell-specific heterochromatin.

Genome research·2026
Same journal

Telomere-driven replicative crisis is driven by large-scale changes in genomic architecture.

Genome research·2026
Same journal

Spatially informed reference-free cell-type deconvolution for spatial transcriptomics with SpatialCD.

Genome research·2026
See all related articles

This study introduces novel high-performance liquid chromatography (HPLC) arrays for mutation detection using heteroduplex analysis. These arrays enable rapid and efficient DNA fragment analysis, improving mutation identification.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Mutation detection is crucial for genetic analysis and disease diagnosis.
  • Existing methods for DNA mutation analysis can be time-consuming and complex.
  • High-performance liquid chromatography (HPLC) offers potential for sensitive and rapid separations.

Purpose of the Study:

  • To demonstrate the feasibility of monolithic poly(styrene-divinylbenzene) capillary HPLC arrays for mutation detection.
  • To develop an efficient method for heteroduplex analysis under partially denaturing conditions.
  • To enhance throughput and versatility in DNA fragment analysis.

Main Methods:

  • Utilized novel monolithic poly(styrene-divinylbenzene) capillary columns (0.2 mm internal diameter).

Related Experiment Videos

  • Employed partially denaturing conditions for heteroduplex analysis.
  • Explored simultaneous analysis of samples at different temperatures or different samples at the same temperature.
  • Incorporated laser-induced fluorescence detection and differential fluorophore labeling.

    • Main Results:

    • Successfully constructed HPLC arrays for mutation detection via heteroduplex analysis.
    • Demonstrated the ability to analyze single samples at multiple temperatures or multiple samples simultaneously.
    • Achieved analysis times of a few minutes with extremely short turnaround times.
    • Highlighted advantages over capillary electrophoresis, including chemical inertness and robust column beds.

    Conclusions:

    • Monolithic capillary HPLC arrays represent a significant advancement for mutation detection.
    • The developed method offers high throughput, efficiency, and versatility in genetic analysis.
    • This technology provides a robust and rapid alternative to existing mutation detection techniques.