Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A New Realization of SI for Organic Chemical Measurement: NIST PS1 Primary Standard for Quantitative NMR (Benzoic Acid).

Analytical chemistry·2018

Same author

The Effect of Urine Concentration and pH on the Growth of Escherichia Coli in Canine Urine In Vitro.

Journal of veterinary internal medicine·2018

Same author

Physical and Kinetic Properties of Carbamyl Phosphate Synthetase from Frog Liver.

The Journal of biological chemistry·2015

Same author

Assessment of nutrient supplement to reduce gentamicin-induced ototoxicity.

Journal of the Association for Research in Otolaryngology : JARO·2014

Same author

Large-area nanopatterning of self-assembled monolayers of alkanethiolates by interferometric lithography.

Langmuir : the ACS journal of surfaces and colloids·2010

Same author

Backward stimulated Raman scattering in multimode fiber.

Applied optics·2010

Search research articles

Related Experiment Video

Updated: Jul 19, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Robust and efficient synthetic method for forming DNA microarrays.

P L Dolan¹, Y Wu, L K Ista

¹Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA.

Nucleic Acids Research

|November 3, 2001

Summary

Researchers developed a new DNA microarray method using p-aminophenyl trimethoxysilane (ATMS)/diazotization chemistry. This technique enables sensitive gene expression analysis with reusable slides and improved DNA binding.

More Related Videos

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

Related Experiment Videos

Last Updated: Jul 19, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

Area of Science:

Biotechnology
Molecular Biology
Genomics

Background:

DNA microarray technology is crucial for measuring global gene expression patterns.
Current methods require interdisciplinary collaboration for efficient gene expression analysis.
Improving DNA immobilization on glass slides is key to enhancing microarray performance.

Purpose of the Study:

To develop a chemically reactive surface for covalent DNA binding on glass slides.
To improve cDNA microarray technology through enhanced DNA immobilization.
To create reusable microarrays with sensitive detection capabilities.

Main Methods:

Fabrication of microarrays using p-aminophenyl trimethoxysilane (ATMS)/diazotization chemistry.
Analysis of DNA spot uniformity and quantity compared to commercial techniques.
Hybridization analysis to assess sensitivity and background fluorescence.

Main Results:

The ATMS/diazotization method resulted in uniform DNA spots with equivalent or greater DNA amounts than commercial methods.
Hybridization analyses demonstrated 2-3 orders of magnitude greater sensitivity for target sequence detection.
Minimal DNA loss during repeated stripping and re-hybridization confirmed slide reusability.

Conclusions:

ATMS/diazotization chemistry facilitates covalent binding of unmodified DNA to glass slides.
The developed reusable microarrays exhibit enhanced hybridization sensitivity and low background fluorescence.
This advancement offers a more efficient and sensitive platform for gene expression profiling.