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Fusion of Secretory Vesicles with the Plasma Membrane

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Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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Related Experiment Video

Updated: Jul 11, 2026

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Published on: October 19, 2012

Cell biology. Fusion without SNAREs?

S J Scales¹, M F Finley, R H Scheller

¹Genentech Inc., South San Francisco, CA 94080, USA. sscales@gene.com

Science (New York, N.Y.)

|November 3, 2001

Summary

Molecular rearrangements drive membrane fusion, crucial for processes like neurotransmitter release. Scales et al. review new findings on the protein interactions governing this complex cellular event.

Area of Science:

Molecular biology
Cellular neuroscience

Background:

Membrane fusion is essential for cellular functions, including neurotransmitter release at synapses.
Understanding the protein machinery mediating membrane fusion is a key challenge in cell biology.

Discussion:

Scales et al. provide an overview of recent research on protein interactions in membrane fusion.
The reviewed studies by Schoch et al. and Wang et al. offer novel insights into the molecular mechanisms.

Key Insights:

Specific protein interactions are critical for orchestrating the complex molecular rearrangements during membrane fusion.
The research highlights the intricate regulatory networks governing membrane fusion events.

Outlook:

Further investigation into these protein interactions will advance our understanding of synaptic function and related disorders.

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Last Updated: Jul 11, 2026

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

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Published on: October 19, 2012

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

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Published on: August 24, 2016

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

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Published on: December 8, 2019

This work paves the way for potential therapeutic strategies targeting membrane fusion processes.