Related Experiment Video
Updated: Aug 9, 2026

Measurement of Heme Synthesis Levels in Mammalian Cells
Published on: July 9, 2015
[Hemoglobin-induced lipid peroxidation in anemia]
In iron-deficiency anemia (IDA), lower hemoglobin (Hb) levels correlate with increased methemoglobin (Met-Hb). Elevated Met-Hb is linked to heightened lipid peroxidation products in red blood cells.
Area of Science:
- Biochemistry
- Hematology
- Anesthesiology
Background:
- Iron-deficiency anemia (IDA) is a common condition affecting oxygen transport.
- Hemoglobin (Hb) derivatives like methemoglobin (Met-Hb) and carboxyhemoglobin (CO-Hb) can alter red blood cell function.
- Lipid peroxidation (LPO) is a marker of oxidative stress.
Purpose of the Study:
- To investigate the relationship between hemoglobin levels, its derivatives, and lipid peroxidation processes in patients with IDA.
- To explore the role of Met-Hb and CO-Hb in the activation of LPO in IDA.
Main Methods:
- Studied peroxide oxidation of lipids (neutral lipids and phospholipids) in 33 patients with IDA.
- Measured levels of hemoglobin, methemoglobin, and carboxyhemoglobin in erythrocytes and blood plasma.
- Correlated Hb derivative levels with LPO products.
Main Results:
- A significant negative correlation was found between Hb concentration and Met-Hb levels (r = -0.670, P = 0.038).
- Increased Met-Hb levels correlated with accumulation of Schiff bases (phospholipids peroxidation products) (r = 0.586, P = 0.004).
- Met-Hb and CO-Hb levels correlated with primary and secondary products of neutral lipid peroxidation, and CO-Hb with LPO substrates.
Conclusions:
- Hemoglobin derivatives, particularly Met-Hb, play a role in activating lipid peroxidation in iron-deficiency anemia.
- The findings suggest a mechanism involving Met-Hb and CO-Hb in the oxidative stress associated with IDA.
More Related Videos
05:17Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
Published on: July 28, 2016
07:29Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Related Concept Videos
Necrosis
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become anucleated and die, but their...
Hemoglobin
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
Disorders of Erythrocytes
Erythrocyte disorders can be broadly categorized into two main types: anemic and polycythemic conditions.
A low oxygen-carrying capacity of the blood due to the loss, lower production, or destruction of erythrocytes is termed anemia. Hemorrhagic anemia, for example, occurs when bleeding from an external wound or internal ulcer reduces erythrocyte counts.
On the other...
Factors Affecting Erythropoiesis
Several factors influence the erythrocyte production rate, with tissue oxygen level being among the most critical. Intense exercise or high altitudes can cause tissue hypoxia, which triggers the kidneys to release more erythropoietin (EPO) into the bloodstream.
EPO then...
Oxygen Transport in the Blood
Hypoxia
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...