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Endocardial expression and functional characterization of endothelin-1.

O Saetrum Opgaard¹, M Adner, T H Peters

¹Department of Internal Medicine, University Hospital, Lund, Sweden.

Molecular and Cellular Biochemistry

|November 6, 2001

Summary

Endocardial endothelial cells produce and release active Endothelin-1 (ET-1), a peptide hormone. This ET-1 influences myocardial contractility and may act on other organs.

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Area of Science:

Cardiovascular Biology
Endocrinology
Cell Biology

Background:

Endothelin-1 (ET-1) is a peptide with significant vasoconstrictive and cardiac effects.
The expression and function of ET-1 in endocardial endothelial cells are not fully understood.

Purpose of the Study:

To investigate the presence and functional activity of ET-1 in human endocardial endothelial cells.
To determine the role of ET-1 in regulating myocardial contractility.

Main Methods:

Immunofluorescence localization to detect ET-1 protein.
RT-PCR to analyze ET-1 and its receptor mRNA expression.
Organ bath studies using rat aortic segments to assess biological activity.

Main Results:

ET-1 was detected in the cytoplasm of human endocardial endothelial cells.
ET-1, ET(A), and ET(B) receptor mRNAs were expressed in myocardial and endocardial cells.
Conditioned media from endothelial cells induced sustained contractions in aortic segments, blocked by an ET(A) receptor antagonist.

Conclusions:

Human endocardial endothelial cells express and release biologically active ET-1.
Endocardial ET-1 likely plays a role in regulating myocardial contractility.
ET-1 released by endothelial cells may function as a circulatory peptide affecting peripheral organs.