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Published on: January 7, 2019
Influence of 7beta-hydroxycholesterol on glutathione status and nitric oxide production in macrophages: in vitro
G Bansal1, U Singh, M P Bansal
1Department of Biophysics, Panjab University, Chandigarh, India.
Abstract:
Incubation of murine peritoneal macrophages with 7beta-hydroxycholesterol (7beta-OH) for 24 hr led to dose-dependent reduction in cellular glutathione content as well as nitrite levels in the medium. Treatment with an inorganic form of selenium, sodium selenite which is a potent antioxidant, elevated the cellular glutathione levels and decreased nitrite levels. Our results suggest that 7beta-OH may exert its pro-atherogenic effect by inhibiting glutathione synthesis and nitric oxide production by macrophages present in the arterial wall and thus, impair the cellular antioxidant defense system.

