Investigation of the anthracene-nitroxide hybrid molecule as a probe for hydroxyl radicals
Abstract:
A new method for the determination of hydroxyl radicals is proposed. The method is based on the use of a hybrid molecule consisting of a fluorescent chromophore, anthracene, and a nitroxide radical. In the hybrid molecule, the nitroxide quenches the fluorescence of anthracene strongly. The reaction of hydroxyl radicals with dimethyl sulfoxide generates quantitatively methyl radicals, which then combine with the nitroxide moiety of the hybrid molecules to result in an increase in the fluorescence intensity. The fluorescence increase is proportional to the concentration of hydroxyl radicals. The proposed method is capable of detecting hydroxyl radicals generated in the Fenton system. It is a simple and sensitive technique for the determination of hydroxyl radicals.
More Related Videos
13:21Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
Published on: August 18, 2012
13:41Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting
Published on: October 17, 2011
Related Concept Videos
Radical Autoxidation
Radical Reactivity: Nucleophilic Radicals
Oxidation of Alkenes: Anti Dihydroxylation with Peroxy Acids
Radical Anti-Markovnikov Addition to Alkenes: Mechanism
The mechanism starts with chain initiation, which involves two steps. In the first chain initiation step, a weak peroxide bond is homolytically cleaved upon mild heating to form two alkoxy radicals. In the second initiation step, a hydrogen atom is abstracted by the alkoxy...
Radical Reactivity: Overview
Radical Formation: Homolysis
