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Enantiomeric separation and quantitative determination of propranolol in tablets by chiral high-performance liquid
M I Santoro1, H S Cho, E R Kedor-Hackmann
1Departamento de Farmácia, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brasil.
Abstract:
This work reports an application of chiral high-performance liquid chromatography (HPLC) in the separation and quantitative determination of propranolol isomers in tablets. The isomers were separated using a Chiralcel OD column (250 x 4.6 mm, 10 microm) with a mobile phase of hexane:ethanol (75:25 v/v) at a flow rate of 0.7 ml/min and ultraviolet detection at 280nm. The coefficient of variation and average recovery of (R)-isomer for samples A, B, C, and D were 0.72% and 100.30%, 0.67% and 99.40%, 0.62% and 99.76%, and 0.70% and 99.68%, respectively. The coefficient of variation and average recovery of (S)-isomer for samples A, B, C, and D were 0.74% and 99.62%, 0.64% and 100.27%, 0.71% and 99.99%, and 0.70% and 99.72%, respectively.