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Multipulse feed strategy for glycerol fed-batch fermentation: a steady-state nonlinear optimization approach.

D Xie1, D Liu, H Zhu

  • 1Institute of Applied Chemistry, Department of Chemical Engineering, Tsinghua University, Beijing, China. xiedm@tsinghua.edu.cn

Applied Biochemistry and Biotechnology
|November 6, 2001
PubMed
Summary

A novel multipulse feed strategy using nonlinear optimization significantly improved glycerol production during fed-batch fermentation. This method controls residual glucose levels effectively, outperforming previous experimental optimization techniques.

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Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Fermentation Technology

Background:

  • Fed-batch fermentation is crucial for maximizing product yield.
  • Controlling substrate feeding is key to optimizing fermentation processes.
  • Glycerol production requires precise management of glucose and nutrient supply.

Purpose of the Study:

  • To develop and validate a multipulse feed strategy for glycerol production.
  • To maximize glycerol productivity using nonlinear optimization.
  • To maintain low residual glucose concentrations during fermentation.

Main Methods:

  • Dividing the fermentation process into equal subintervals.
  • Implementing pulse-wise feeding of glucose and corn steep slurry.
  • Employing a general nonlinear optimization approach based on macrokinetic models.

Related Experiment Videos

  • Conducting experiments in a 600-mL Airlift Loop Reactor.

    • Main Results:

    • The multipulse feed strategy aligned well with model predictions.
    • Glycerol productivity was significantly enhanced compared to previous methods.
    • Residual glucose levels were effectively controlled at low concentrations.
    • The nonlinear optimization approach proved superior to limited experimental optimization.

    Conclusions:

    • The developed multipulse feed strategy is effective for enhancing glycerol production.
    • Nonlinear optimization provides a robust method for controlling fed-batch fermentation.
    • This approach offers significant improvements over traditional feeding strategies.