Influence of the polymeric morphology of sorbents on their properties in affinity chromatography

The influence of the polymeric morphology of different types of Fe(3+)-containing sorbents and their properties in retention of phosphoamino acids is presented in this paper. Poly(hydroxylated polybutadienic-hydroxyethyl methacrylate) [poly(PB-HEMA)] and poly(ethylene glycol dimethacrylate-hydroxyethyl methacrylate) [poly(EGDMA-HEMA)] base supports were submitted to chemical modifications to attain metal ion-containing sorbents. Properties such as specific surface area, pore volume, equilibrium volume swelling ratios, extent of conversion rate of functional groups, amount of chelated metal ion, ligand occupation, as well as quantity of phosphoamino acid retained, were used as comparative parameters for those different base matrices. Results suggest that Fe(3+) immobilized on poly(EGDMA-HEMA) base support are more efficient as a group-specific sorbent to retain phosphoamino acids than those obtained using poly(PB-HEMA) base support.