Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Affinity purification of DNA-binding proteins.

H Gadgil1, S A Oak, H W Jarrett

  • 1Department of Biochemistry, University of Tennessee, 858 Madison Avenue, Memphis, TN 38163, USA.

Journal of Biochemical and Biophysical Methods
|November 6, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Purification and characterization of transcription factors.

Mass spectrometry reviews·2013
Same author

DNA affinity chromatography.

Molecular biotechnology·2001
Same author

Mouse alpha1-syntrophin binding to Grb2: further evidence of a role for syntrophin in cell signaling.

Biochemistry·2001
Same author

Comparative studies on discrete and concatemeric DNA-sepharose columns for purification of transcription factors.

Journal of chromatography. A·2001
Same author

DNA affinity chromatography of transcription factors.

Analytical biochemistry·2001
Same author

Bi-column method for purification of transcription factors.

Journal of chromatography. A·2001

DNA affinity chromatography is a powerful technique for purifying DNA binding proteins. This review covers strategies using various DNA affinity columns for diverse proteins and applications.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Protein Purification

Background:

  • DNA binding proteins play crucial roles in cellular processes.
  • Efficient purification methods are essential for studying these proteins.
  • DNA affinity chromatography offers a powerful approach for protein isolation.

Purpose of the Study:

  • To review the utility of DNA affinity chromatography for purifying DNA binding proteins.
  • To discuss strategies employing different types of DNA affinity columns.
  • To highlight diverse applications of this purification technique.

Main Methods:

  • Utilizing nonspecific DNA affinity columns.
  • Employing sequence-specific DNA affinity columns.
  • Using single-stranded DNA affinity columns for protein purification.

Related Experiment Videos

Main Results:

  • Detailed purification strategies for transcription factors, restriction enzymes, telomerases, DNA and RNA polymerases, and DNA binding antibodies are presented.
  • Demonstration of the effectiveness of DNA affinity chromatography across various protein classes.
  • Showcasing the versatility and broad applicability of the technique.

Conclusions:

  • DNA affinity chromatography is a highly effective method for purifying DNA binding proteins.
  • The choice of DNA affinity column can be tailored to specific protein targets.
  • This technique facilitates significant advancements in molecular biology research.