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Updated: Aug 1, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
Affinity purification of DNA-binding proteins
H Gadgil1, S A Oak, H W Jarrett
1Department of Biochemistry, University of Tennessee, 858 Madison Avenue, Memphis, TN 38163, USA.
Abstract:
The focus of this review is on DNA affinity chromatography, which is the most powerful tool for purification of DNA binding proteins. The use of nonspecific-, sequence specific- and single stranded-DNA affinity columns in purification of various DNA binding proteins is discussed. The purification strategies for transcription factors, restriction enzymes, telomerases, DNA and RNA polymerase and DNA binding antibodies are described. Different applications of DNA affinity chromatography are presented.
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