Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CB6F1-rasH2 mouse: overview of available data.

T Usui1, M Mutai, S Hisada

  • 1Central Institute for Experimental Animals, Kawasaki, Japan. usui@ciea.or.jp

Toxicologic Pathology
|November 7, 2001
PubMed
Summary

The rasH2 transgenic mouse model effectively identified genotoxic carcinogens and showed a low false-positive rate for non-genotoxic compounds in carcinogenicity testing. This model shows promise for alternative toxicity testing.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Changes in the levels of mRNAs for putative cell growth-related genes in the albedo and flavedo during citrus fruit development.

Plant cell reports·2019
Same author

A Green Journal Initiative.

Veterinary pathology·2017
Same author

A special focus on diseases of aquatic animals.

Veterinary pathology·2013
Same author

Should we kill the case report?

Veterinary pathology·2008
Same author

The novel microtubule-interfering agent TZT-1027 enhances the anticancer effect of radiation in vitro and in vivo.

British journal of cancer·2007
Same author

Adaptation of the WHO guideline for residual DNA in parenteral vaccines produced on continuous cell lines to a limit for oral vaccines.

Developments in biologicals·2006

Area of Science:

  • Toxicology
  • Genetics
  • Carcinogenesis

Background:

  • The International Life Sciences Institute's (ILSI) Health and Environmental Sciences' (HESI) Alternative to Carcinogenicity Testing (ACT) project evaluated the CB6F1-rasH2 transgenic mouse.
  • This study includes data from short-term carcinogenicity assessments using this model, alongside comparable external studies.

Purpose of the Study:

  • To assess the utility of the rasH2 transgenic mouse model in carcinogenicity testing.
  • To evaluate the model's performance in detecting genotoxic and non-genotoxic carcinogens.

Main Methods:

  • Short-term carcinogenicity studies were conducted using the CB6F1-rasH2 transgenic mouse.
  • Compounds tested included genotoxic carcinogens, non-genotoxic rodent carcinogens, human carcinogens, hormones, and non-carcinogens.

Related Experiment Videos

  • Standardized and modified protocols were employed across participating laboratories.

    • Main Results:

    • 3 of 4 genotoxic carcinogens tested were positive in the rasH2 mouse; one was equivocal.
    • The positive control N-Methyl-N-nitrosurea (MNU) showed reproducible positive responses.
    • Two peroxisome proliferators (clofibrate, DEHP) were positive among non-genotoxic rodent carcinogens; other non-genotoxic non-carcinogens were negative.

    Conclusions:

    • The rasH2 mouse model demonstrated efficacy in identifying genotoxic carcinogens.
    • The model showed a low false-positive rate for non-genotoxic compounds, suggesting its reliability.
    • These findings support the rasH2 mouse as a valuable tool for alternative carcinogenicity testing.