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Updated: Oct 3, 2026

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
Published on: April 5, 2019
Anion selective exhaustive injection-sweep-micellar electrokinetic chromatography
1Faculty of Science, Himeji Institute of Technology, Kamigori, Hyogo, Japan. jbkim@sci.himeji-tech.ac.jp
Abstract:
A selective on-line sample concentration technique of anion selective exhaustive injection (ASEI)-sweep-micellar electrokinetic chromatography (MEKC) was evaluated using a cationic surfactant. To suppress the electroosmotic flow, a polyacrylamide-coated capillary was introduced. Some aromatic carboxylic acids, dansyl amino acids, and naphthalene-disulfonic acids were used as test analytes. About 1,000- to 6,000-fold increases in detection sensitivity were obtained in terms of peak heights by ASEI-sweep-MEKC.
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