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Updated: Aug 10, 2026
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
A rapid, non-destructive method for the determination of Staphylococcus epidermidis adhesion to surfaces using quartz
K D Pavey1, L M Barnes, G W Hanlon
1School of Pharmacy and Biomolecular Sciences, Cockcroft Building, University of Brighton, Lewes Road, Brighton, BN2 4GJ, UK. K.D.Pavey@bton.ac.uk
Aims:
To investigate the use of quartz crystal resonant sensor (QCRS) technology to determine the adhesion of Staphylococcus epidermidis to fibronectin-coated surfaces.
Methods And Results:
QCRS sensors (14 MHz) with 4 mm gold electrodes were coated with fibronectin and exposed for 15 min to suspensions of Staph. epidermidis ranging in concentration from 1 x 10(2) to 1 x 10(6) cfu ml(-1). Changes in resonant frequency were recorded and showed a linear relationship with the logarithm of cell concentration over the range tested.
Conclusions:
QCRS technology was shown to be a rapid, sensitive and non-destructive method for measuring the adhesion of bacteria to surfaces.
Significance And Impact Of The Study:
This report demonstrates that QCRS technology has the potential to be used for a range of applications requiring measurement of bacteria on surfaces. In particular, it may be used for the real-time monitoring of bacterial biofilm formation.
