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Identification of F. o. cucumerinum and F. o. luffae by RAPD-generated DNA probes
1Department of Microbiology, Soochow University, Taipei, Taiwan, R.O.C. phwang@mail.scu.edu.tw
Aims:
To create a fast, sensitive and specific method for identifying Fusarium oxysporum f. sp. cucumerinum and F. o. luffae.
Methods And Results:
Specific DNA bands were selected as probes from RAPD profiles of 13 formae speciales of F. oxysporum. The forma specialis-specific probe OPC18300c and OPC18520f could be used to identify F. o. cucumerinum and F. o. luffae by RAPD-PCR followed dot blot hybridization, respectively.
Conclusions:
A specific method for identifying F. o. cucumerinum and F. o. luffae was achieved.
Significance And Impact Of The Study:
F. oxysporum formae speciales identification with a DNA probe can be relatively rapid and provides a method to identify the pathogen without host inoculation tests.