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Related Experiment Videos

Tyrosine nitration: localisation, quantification, consequences for protein function and signal transduction.

S A Greenacre1, H Ischiropoulos

  • 1Centre for Cardiovascular Biology and Medicine and Wolfson Centre for Age-related Disease, King's College London, Guy's Campus, London, SE1 1UL, UK.

Free Radical Research
|November 8, 2001
PubMed
Summary

3-nitrotyrosine, a marker of reactive nitrogen species, is formed under physiological conditions and increases in disease. Its detection and localization in tissues and proteins are crucial for understanding disease pathogenesis and protein function.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pathology

Background:

  • Tyrosine nitration generates 3-nitrotyrosine, a marker for reactive nitrogen species like peroxynitrite.
  • Detection of 3-nitrotyrosine indicates tyrosine nitration occurs in vivo under physiological conditions.
  • Elevated 3-nitrotyrosine levels are observed in most disease states.

Purpose of the Study:

  • To provide a comprehensive summary of 3-nitrotyrosine localization and quantification.
  • To examine the consequences of protein nitration on protein function and disease.
  • To explore the effects of protein nitration on signal transduction and protein metabolism.

Main Methods:

  • Analytical techniques for 3-nitrotyrosine detection.
  • Immunological techniques for 3-nitrotyrosine detection.

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  • Review of existing literature on 3-nitrotyrosine localization and quantification.

    • Main Results:

    • 3-nitrotyrosine is localized in specific tissues, cells, and proteins.
    • Quantification methods for 3-nitrotyrosine have been established.
    • Tyrosine nitration impacts protein function, signal transduction, and metabolism.

    Conclusions:

    • 3-nitrotyrosine serves as a valuable biomarker for oxidative/nitrosative stress.
    • Protein nitration plays a significant role in the pathogenesis of various diseases.
    • Understanding protein nitration is essential for developing therapeutic strategies.