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ADAM15 overexpression in NIH3T3 cells enhances cell-cell interactions
B Herren1, K J Garton, S Coats
1Department of Pathology, University of Washington School of Medicine, Seattle, Washington 98104-2499, USA.
Experimental Cell Research
|November 8, 2001
Summary
Overexpression of ADAM15 (a metalloprotease-disintegrin) enhances cell-cell interactions, leading to decreased cell migration and increased cell adhesion in NIH3T3 cells.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- ADAM15 belongs to the metalloprotease-disintegrin family, known for integrin interactions.
- The role of ADAM15 in regulating cell-matrix and cell-cell interactions requires further elucidation.
Purpose of the Study:
- To investigate the impact of ADAM15 overexpression on NIH3T3 cell behavior.
- To determine the specific effects on cell migration, adhesion, and cell-cell interactions.
Main Methods:
- Utilized tetracycline-regulated ADAM15 overexpression in NIH3T3 cells.
- Performed Boyden chamber assays and scratch wound models to assess cell migration.
- Analyzed cell adhesion, monolayer permeability, and cell morphology.
- Investigated ADAM15 localization in epithelial cells.
Main Results:
- ADAM15 overexpression inhibited NIH3T3 cell migration on fibronectin.
- No significant changes were observed in matrix attachment or ERK signaling.
- Monolayer permeability decreased, and cell morphology altered, suggesting increased cell-cell interactions.
- Cell adhesion to ADAM15-overexpressing cells increased by 45%.
Conclusions:
- ADAM15 overexpression enhances cell-cell interactions in NIH3T3 cells.
- This enhancement is evidenced by reduced cell migration and increased cell adhesion.
- ADAM15 localizes to cell-cell contacts, supporting its role in cell-cell adhesion.
