Changes in the performance of schedule-induced polydipsia (SIP) in rats after arecoline and amphetamine treatments

This study investigated the performance of schedule-induced polydipsia (SIP) rats in novel or intermittent-reward SIP sessions after arecoline (AREC) and amphetamine sulfate (AMPH) treatments. Either automatic monitors or observers extensively examined the functional changes of parameters in behavioral performance followed by increasing drug dosage. The parameters included locomotion and stereotyped behaviors in the novel sessions; schedule-induced licks, water intake, schedule-dependent nose-pokes, pellets earned and stereotyped behaviors of the facultative stage in the SIP sessions. It was found that when the rats received AMPH (0.5 - 2.0 mg/kg) but not AREC (0.1 - 1.6 mg/kg) in the novel sessions, locomotion increased in a dose-dependent manner. However, when AREC (0.8 mg/kg) and AMPH (1.0 mg/kg) were both given, the effect of AMPH on locomotion was significantly attenuated. In the SIP sessions, a single injection of AMPH increased the number of schedule-dependent nose-pokes at a dose of 1.0 mg/kg, whereas it decreased the number of schedule-induced licks and the amount of water intake at a dose of 2.0 mg/kg. On the other hand a single injection of AREC caused no operant behavior changes at doses below 0.8 mg/kg. However, when the dose was increased to over 0.8 mg/kg (1.6 mg/kg), the number of schedule-induced licks and water intake increased, but the number of schedule-induced nose-pokes decreased. The effects of large doses of AREC on SIP were attenuated after co-administration of scopolamine (0.1 mg/kg), a muscarinic receptor antagonist. Furthermore, the effects of AMPH on SIP performance were not changed by co-administration of AREC at a dose of 0.8 mg/kg. These results are discussed based on the hypothesis that combined utilization of the main component in chewing betel quid, AREC, and AMPH may yield changes of AMPH-induced psychomotor responses in a special environmental context.