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Ribonucleotide reductase activity during amphibian development.

N Tondeur-Six, R Tencer, J Brachet

    Biochimica Et Biophysica Acta
    |June 2, 1975
    PubMed
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    Ribonucleotide reductase activity is crucial for early embryonic development in Xenopus and Pleurodeles. This enzyme

    Area of Science:

    • Developmental Biology
    • Biochemistry
    • Molecular Biology

    Background:

    • Ribonucleotide reductase is essential for DNA synthesis.
    • Its role during early amphibian development is not well understood.

    Purpose of the Study:

    • To investigate the presence and regulation of ribonucleotide reductase during oocyte maturation and early embryonic development in Xenopus and Pleurodeles.

    Main Methods:

    • Enzyme activity assays using [3H] uridine incorporation into DNA.
    • Hormonal induction of oocyte maturation.
    • Inhibition studies with hydroxyurea, cycloheximide, and actinomycin D.

    Main Results:

    • Ribonucleotide reductase activity is absent in mature oocytes but present in unfertilized eggs.

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  • Enzyme synthesis/activation occurs during oocyte maturation and peaks at the 2-4 cell stage.
  • Activity declines during later embryonic stages and is absent in tadpoles.

    • Conclusions:

    • Ribonucleotide reductase is dynamically regulated during early amphibian development.
    • Enzyme synthesis is protein-dependent and transcription-independent during early cleavage.
    • The enzyme plays a critical role in DNA synthesis for rapid cell division in early embryos.