Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sample purification on a microfluidic device.

T Footz1, S Wunsam, S Kulak

  • 1Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Canada.

Electrophoresis
|November 10, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Voltage CMOS Controller for Microfluidics.

IEEE transactions on biomedical circuits and systems·2013
Same author

Microfluidic approach to genotyping human platelet antigens.

IET nanobiotechnology·2012
Same author

Assessing the performance and longevity of Nb, Pt, Ta, Ti, Zr, and ZrO₂-sputtered Havar foils for the high-power production of reactive [18F]F by proton irradiation of [18O]H2O.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2011
Same author

Shah convolution fourier transform detection.

Analytical chemistry·2011
Same author

Integrated circuit-based instrumentation for microchip capillary electrophoresis.

IET nanobiotechnology·2010
Same author

Miniaturized system for isotachophoresis assays.

Lab on a chip·2010
Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
See all related articles

This study introduces a simple microchip-based method for purifying samples, overcoming a major hurdle in microfluidic device implementation. The technique effectively removes contaminants like primers from amplified DNA, enhancing microfluidic capabilities.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Microfluidics

Background:

  • Macroscopic sample preparation methods (precipitation, centrifugation, membranes) are difficult to integrate into microfluidic systems.
  • Existing microfluidic sample purification systems can be complex and hinder widespread adoption.
  • Efficient sample preparation is crucial for the successful implementation of microfluidic devices.

Purpose of the Study:

  • To develop a simplified microchip-based sample purification method compatible with basic microfluidic designs.
  • To demonstrate the removal of specific contaminants from DNA samples using the developed technique.

Main Methods:

  • A novel microchip-based purification technique was developed.
  • The method was demonstrated by removing primers from amplified DNA samples.

Related Experiment Videos

  • The technique is designed for integration into simple microfluidic architectures.

    • Main Results:

    • The microchip-based method successfully purified amplified DNA by removing primers.
    • The technique is effective even with the simplest microfluidic designs.
    • This method enhances the sample preparation capabilities of microfluidic systems.

    Conclusions:

    • A straightforward and effective microchip-based sample purification method has been established.
    • This technique addresses a significant barrier to implementing macroscopic protocols on microfluidic devices.
    • The developed method offers a new capability for sample preparation in microfluidics.