Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Two-photon excitation fluorescence microscopy.

P T So1, C Y Dong, B R Masters

  • 1Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. ptso@mit.edu

Annual Review of Biomedical Engineering
|November 10, 2001
PubMed
Summary

Two-photon fluorescence microscopy offers advanced 3D biological imaging with submicrometer resolution. This technique reduces photodamage and improves imaging depth for biological and medical applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Cumulative live birth rates after repeat IVF cycles in women with diminished ovarian reserve: a 7-year retrospective cohort of 3 740 patients].

Zhonghua fu chan ke za zhi·2026
Same author

[Effects of electrospun collagen nanofibrous matrix on the biological behavior of human dental pulp cells].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2019
Same author

[Analysis on influencing factors of deaths from severe heat stroke in Shanghai, 2013-2017].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2019
Same author

[A multicenter study of reference intervals for 15 laboratory parameters in Chinese children].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2018
Same author

[Study on the current situation of China's First List of Rare Diseases based on 15 million hospitalizations].

Zhonghua yi xue za zhi·2018
Same author

[Acute effect of ambient air pollution on small airway lung functions among school children in Shanghai].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2017

Area of Science:

  • Biological Imaging
  • Microscopy Technology

Background:

  • Two-photon fluorescence microscopy is a key innovation in biological imaging.
  • It allows for noninvasive, three-dimensional studies of specimens.

Observation:

  • Two-photon excitation involves the simultaneous absorption of two photons by fluorophores.
  • This process offers unique advantages over traditional methods.

Findings:

  • Reduced specimen photodamage and enhanced penetration depth.
  • Higher-contrast images and localized photochemical reaction triggering.
  • Successful application in diverse biological and medical fields.

Implications:

  • Enables deeper and less damaging visualization of biological structures.

Related Experiment Videos

  • Facilitates novel research in cell biology, neuroscience, and medicine.
  • Drives advancements in diagnostic and therapeutic imaging techniques.