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Updated: Oct 3, 2026

Essential Components of Borreliella (Borrelia) burgdorferi In Vitro Transcription Assays
Published on: July 22, 2022
Enzyme activities of Borrelia burgdorferi sensu lato
M Heroldová1, M Nĕmec, Z Hubálek
1Department of Microbiology, Faculty of Science, Masaryk University, 602 00 Brno, Czechia.
Abstract:
Activities of 19 enzymes were tested by the API ZYM system in 13 strains of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto, B. afzelii, B. garinii, B. lusitaniae, B. valaisiana) grown in liquid BSK-H medium supplemented with rabbit serum. All strains produced acid phosphatase, esterase (C4), esterase-lipase (C8), leucine arylamidase and naphthol-AS-BI-phosphohydrolase. Nine strains also produced alkaline phosphatase, and three strains produced alpha-glucosidase. The API ZYM system probably cannot be used for differentiation between B. burgdorferi sensu lato genomospecies.
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