Related Experiment Video
Updated: Oct 3, 2026
Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining
Published on: December 23, 2016
[Tissue tropism of novel nonenveloped DNA virus in experimentally infected rhesus monkey]
1Nanfang Hospital, Affiliated to First People's Liberation Army Medical University, Guangzhou 510515, China.
Objective:
To investigate the tissue tropism of transfusion transmitted virus (TTV), a novel nonenveloped DNA virus, in experimentally infected Rhesus monkey.
Methods:
Filtrate of feces of one patient with TTV infection was fed into the stomachs of ten Rhesus monkeys. Five (?) monkeys were killed during viremia and their tissues were collected to be examined for the presence of replicative virus DNA by in situ hybridization, dot blot hybridization with antisense probe and hybridization/nuclease protection assay.
Results:
The virus contents (density of electrophoretic band) in the liver, PBMC, and serum of three infected monkeys 50.1%, 31.0%, and 18.9% respectively; the relative proportion was 2.65;1.64;1.00. TTV was detected in liver, spleen, stomach, small intestine and its lymph nodes, PBMC, colon, and serum; however, viral plus-chain, a putative replicative intermediate, was found only in liver, spleen, small intestine, and lymphoid cells.
Conclusion:
Transfusion transmitted virus is hepato-, spleno-, and lymphoid cell-tropic, and may has multiple routes of transmission.
