[Expression of extracellular domain of VEGF receptor KDR with recombinant AAV]

New vessel formation plays a key role in tumor growth and transforming, and the Vascular endothelial growth factor (VEGF) is the most important factor inducing tumor vessel formation. The cDNA of extracellular domain of VEGF receptor KDR was cloned from primary cultured HUVEC by RT-PCR and subcloned into AAV vector pSNAV. Recombinant AAV was obtained from BHK cells transformed with pSNAV/KDR after adding helper virus. The recombinant AAV expressing soluble KDR that can bind to VEGF. In vivo experiment demonstrated that the recombinant virus can inhibit the new vessel formation of melanoma in C57 mice model.