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Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids
Published on: September 25, 2016
[Studies on the growth characteristics of Spirulina cells under batch and continuous conditions]
1State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080, China. zengwenlu@hotmail.com
Abstract:
The research paper focused mainly on the growth characteristics of Spirulina cells under batch and continuous conditions. It was found that the Richards and Exponential decay model could well express the relationships between the concentration of the cell, carbon in the medium and culture time respectively. The maximum cell growth rate and the cell yield coefficient against carbon under batch culture was 0.371 g/d/L and 3.439 g/gC respectively. The concentration of cell and that of carbon in the reactor increased and decreased with the dilution rate respectively under the continuous culture condition. The maximum cell growth rate and the optimum dilution rate was 0.362 g/d/L and 0.45/d respectively. The cell yield against carbon under continuous culture condition was 2.050 g/gC. The cell growth rate of batch culture was larger than that of continuous one when the cell concentration was relatively low, but it will be smaller than that of continuous culture when the cell concentration became high.
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