[The package and identifying of hNT-3 recombinated retroviral vector]

¹College of Bioengineering, East China University of Science & Technology, Shanghai 200237, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology

|November 13, 2001

Abstract:

hNT-3 was inserted into retroviral vector pLXSN to get pLXSN-NT3, and it was transfected into packaging cell line PA317 to form G418 resistant cell clones. The G418 resistant clones were titered and checked for the existence of replication viral. Genome DNA isolated from the cell clone of highest titeration showed the function gene, hNT-3 cDNA, had integrated into the genome of host cells verified by PCR. And bioassay of the clone's cell culture supernant exhibited that it can induce neurite over-growth in the primary culatures of rat spinal cord dorsal root ganglions as compared with controls.

Related Experiment Videos

[The package and identifying of hNT-3 recombinated retroviral vector]

Related Concept Videos

First Measurement of the Decay-Time-Integrated CP Asymmetry in B_{s}^{0}→D_{s}^{-}π^{+} Decays.

Study of Nuclear Effects on Charm Production in Light-Ion Collisions.

First Evidence of the Decay B^{+}→π^{+}e^{+}e^{-}.

[Analysis of the prevalence rate and risk factors of gallstone in the elderly in Xuhui District of Shanghai].

CP Violation Analysis of Local and Nonlocal Amplitudes in the B[over ¯]^{0}→K[over ¯]^{*0}μ^{+}μ^{-} Decay.

Search for the Lepton-Flavor Violating Decays B^{+}→π^{+}μ^{±}e^{∓}.

[Teaching reform and practice of a project-based graduate course Bioinformatics in the era of artificial intelligence].

[Teacher team construction and leapfrog development pathways for bioengineering disciplines in industry-oriented universities].

[Construction and practice of a talent training model for synthetic biology].

[Teaching practice of anaerobic digestion microorganisms in Microbiology for Environmental Engineering].

[Decellularized scaffolds derived from porcine testes for culture of primary testicular cells and organoids].

[Construction and characterization of a stable Cas9-expressing monoclonal WSL cell line].

Chitosan-dsRNA improves tissue stability and delivery for RNAi-mediated Varroa destructor control.

Zinc displacement and metabolic interference: cobalt ion-mediated therapy for broad-spectrum CRKP-resistant pneumonia via inhalable microspheres.

Spray Freeze Drying for Solid Formulations of RNA Lipid Nanoparticles.

Impact of transfection optimization on adeno-associated virus purification performance and vector quality.

Energy-based prediction of biological feedstock densification from laboratory to industrial pelleting.

Fabrication of MIL-88B(Co/Fe)/Ti3C2Tx nanocomposite for the vortex-assisted dispersive micro-solid phase extraction of Cu(II), Cd(II), and Pb(II) in edible grains and wastewater via flame atomic absorption spectrometry.

Related Experiment Videos

[The package and identifying of hNT-3 recombinated retroviral vector]

Abstract:

Related Concept Videos

Related Articles