Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cyclin' round the cell with Rac.

A J Ridley1

  • 1Ludwig Institute for Cancer Research, Royal Free and University College Medical School, London, United Kingdom.

Developmental Cell
|November 13, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing the Quality of Models of the Ambient Solar Wind.

Space weather : the international journal of research & applications·2020
Same author

RhoA, RhoB and RhoC have different roles in cancer cell migration.

Journal of microscopy·2013
Same author

Analysis of Rho GTPase expression in T-ALL identifies RhoU as a target for Notch involved in T-ALL cell migration.

Oncogene·2012
Same author

A model of localised Rac1 activation in endothelial cells due to fluid flow.

Journal of theoretical biology·2011
Same author

Regulation of macrophage adhesion and migration by Rho GTP-binding proteins.

Journal of microscopy·2008
Same author

Differential effects of immunosuppressive drugs on T-cell motility.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2006
Same journal

AXIN1 and AXIN2 regulate the WNT-signaling landscape to promote distinct mesoderm programs.

Developmental cell·2026
Same journal

ARID1A terminates gastric regeneration to prevent cancer.

Developmental cell·2026
Same journal

Myc sustains sex-biased organ zonation in the Drosophila intestine.

Developmental cell·2026
Same journal

Two parallel neuronal circuits involving electrical synapse and DAF-7/TGF-β signaling regulate muscle autophagy in C. elegans.

Developmental cell·2026
Same journal

Menstruation: Once unspoken but now uncovered, one cell type at a time.

Developmental cell·2026
Same journal

The ALS- and FTD-associated proteins annexin A11 and CHMP2B act sequentially in plasma membrane repair.

Developmental cell·2026
See all related articles

New research reveals that integrins stimulate cell cycle progression by activating Rac in endothelial cells. This activation, specifically on fibronectin, regulates the translation of Cyclin D1 mRNA, offering new insights into cell growth.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Integrins are crucial cell surface receptors mediating cell adhesion and signaling.
  • Cell cycle progression is a tightly regulated process essential for cell proliferation.
  • Cyclin D1 is a key regulator of the G1/S phase transition in the cell cycle.

Purpose of the Study:

  • To elucidate the role of integrins in regulating endothelial cell cycle progression.
  • To investigate the signaling pathways downstream of integrin activation.
  • To identify the molecular mechanisms by which integrins influence Cyclin D1 expression.

Main Methods:

  • Endothelial cells were cultured on fibronectin and laminin-coated surfaces.
  • Rac activation was measured using pull-down assays.

Related Experiment Videos

  • Cyclin D1 mRNA translation was assessed via Western blotting and quantitative PCR.

    • Main Results:

    • Rac activation was observed in endothelial cells plated on fibronectin but not on laminin.
    • Integrin engagement with fibronectin led to increased Cyclin D1 mRNA translation.
    • Inhibition of Rac activity blocked the fibronectin-induced increase in Cyclin D1 translation.

    Conclusions:

    • Integrin-mediated signaling, particularly on fibronectin, activates Rac.
    • Activated Rac is essential for regulating Cyclin D1 mRNA translation.
    • This pathway represents a novel mechanism by which integrins promote cell cycle progression in endothelial cells.